I believe that Tsuneyo misunderstood what to do in the stand-up comedy contest as well as what jokes are in general.Serious question, was it really a joke? Because it didn't feel that way to me.
I was so embarrased, but what was more weird is that no one really took it serious but maybe Otoha?
More than a few took it seriously. Sensei, Molly, and Tsuneyo where involved in that "joke" and Imani noticed something which is why she pulled Tsuneyo off the stage. Maya knows what's up. Ayane saved the day by putting the rest of the class's attention on her, but at the end, made Sensei confess all of his relationships with other girls to her. Probably more noticed too.