Depending on the engine used by the game, yes.
For Ren'py games, use
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to extract the scripts and edit directly or use the
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to generate the translation files (Using this method, you will need to "load" the translation using
renpy.change_language:
Python:
#startup.rpy example
label splashscreen:
menu:
"English":
$ renpy.change_language(None)
"Português":
$ renpy.change_language("Portuguese")
"Español":
$ renpy.change_language("Spanish")
return