Could start it without any troubles on my private and work pc, with and without steam running.
The only advice I currently have would be to restart the PC, but that's the usual bullshit tech support advice? Running Win 11 here, but I don't see why it shouldn't work on Win 10.
It is amd64 (x64 / 64bit) only though, I guess. So, if you usually run the ...-32.exe, the game might not work. If you run a 32-bit Windows, you might need to make a build with an earlier Ren'Py version, since Ren'Py dropped 32-bit support with 8.0.3.