Other than Maya (most of the anti-NTR crowd don't care about lesbianism as long as they are virgins with males) who has she obviously had sex with? As other people have said even Jade, who has been married for around 20 years, and Bella who has been married previously, haven't actually been shown to have been intimate with their respective husbands so could still arguably be virgins just to appease the anti-NTR crowd. There's nothing to show that Josy has had sex with another guy before MC, and there never will be.
By the way I'm not part of the anti-NTR crowd, I've argued previously that games should be realistic and if you don't choose an LI on a particular playthrough then they should be able to move on from MC and date other guys (we obviously don't need to see them have sex on screen or anything) but DPC is scared of losing patrons from the vocal minority so for example on the throuple route, Sage, Bella and Jill (plus all the side girls) will stay single and not get close to another guy for the rest of the game, which in my opinion is unrealistic but unfortunately it's just the way it is.