Any time
It's strange that a scan of the complete game folder with MalwareBytes and BitDefender return no positives but at execution time, the game is flagged as malware.
I had the game.exe examined by MWB and they found nothing, further analysis of the log put me on the path of one of the (windows) library files.
I then tried if the folder from the previous release would work (assuming there are no major changes in the Ren'Py engine) and presto!
You should be aware of the possible infestation by malware, though.