While I'm learning python, I don't know renpy so this could be just a wild shot in the dark. There are multiple errors most of which related to your layout scripts not firing. I know you said you don't recall, but my first thought is you used a walkthrough mod (not sure if it touches these, but it kinda makes sense it would). I'd suggest what
TheDevian suggested and try an auto save or check out the walkthrough mod and see if it sounds familiar, install it and see if your save starts working again. Otherwise unless someone can give you more precise knowledge of the renpy errors you have, you're likely going to need a new save.