As in the chakra actually has to take hold, change the target in some way, even if it is noticeable to only the most perceptive player/character. If it doesn't, she won't act any different than if she never had chakra forced into her in the first place and would just crush the MC after a 'pitiful effort' to stop her. The very first time you get caught by Anko, the chakra has very little effect on her, only enough that she doesn't kill you where she finds you, which would be right in front of whichever one of your girls you were with at the time. The first time for Tsunade was the same, in fact, so was the transition to level 2. As of that point, she is still openly hostile, she just can't seem to act on it. It's going to take more than what the MC did to them to get Kaguya, possibly nearly all he can handle in one day without using any other jutsu, to even get her to that point. Anything less and he is dead, even if he managed to pour a little in. His chakra isn't all powerful, it takes a minimum amount that varies by the will of each target. That's why Tsunade and Anko didn't fall into a full trance immediately like the others.