It's brought up for comedy several times as people have pointed out, but what people haven't mentioned is that being a brony is red path. You miss out on a lust event by doing so.
I could cite a lot of examples of the same thing happening over the years.
I'll even bring up my recent go at it. In 0.46, Ami and Niki are at her Christmas concert and Ami basically tells Niki that Akira and her have been fucking, and that he's been fucking most of the girls in his class. In the next update there are a bunch of invite events that Niki is a part of, which is unprecedented breaking away from the "girl + Ami have a sfw event" formula.
I notice some oddities in the Sana virginity event, like Niki breaking Japanese protocol draining the bath water early, and a joke that is either how a smart person would respond to a dumb attempt at changing the subject, or it means Niki is actually a fucking idiot.
I thought Niki listened to Ami and was paying attention to the girls Akira was inviting over, left the two of them alone for a while and saw proof that he was fucking his students, then had to make up an excuse for why there wasn't any bath water in the first place.
Then we get the changelogs that were eventually rearranged due to fanfest, and all of a sudden 0.50 is full of heavy hitter names like something big is going down, I theorized there was going to be a big confrontation (and that it somehow ended with Niki setting up Noriko for the D) that would resolve this plotline and the several chapter long joke of Noriko getting cucked.
Instead when it actually happens, Noriko grows a spine and gets the D basically herself, then Niki catches them fucking by chance (steered by Ami sure, but it was a random event that put them in a private location for this to happen in the first place). It turns out Niki completely ignored everything Ami said in 0.46, she was just randomly around for the invite events in 0.47, and that joke in 0.47 actually meant Niki was a fucking idiot.
That said I haven't fully learned my lesson either. I'm still hoping
Otoha gets raped because I think the moment Akira would get thanks to it would resolve Chapter 4 beautifully. (Prime returns on the rooftop with all the other RAS girls surrounding them, Akira rushes to embrace her, some lovely words between them, they share a kiss, screen fades to black, after a while Prime and the others start panicking, we flash to Akira blacking out in Prime's arms with a bunch of panicked girls in the background looking down at them, "Chapter 4 End", and proof that if they had kissed during fireflies he would have been reset.)