No, but you gotta talk to them individually to get their quest or whatever. The choice to choose their side.no need to choice there a pink skill thing that lets u impregnate them all
Anyways I feel like I ask this every update, but what are the possible routes with Rio...? She seems really vanilla to me, even though I completed the eternal contract. I want to mess with her mind a bit but there were not too many opportunities I think.