Well yes, but actually no. You see, in order for her to be pussy fucked she needs to be wet. In order to get wet she needs to have pleasure above 33% I think and then she will get wet in a few turns.Can someone whose subjugated Level 1 do a test for me? Go and see if the thugs will fuck Karryn's pussy. They no longer fuck her in my game for some reason, but they were fucking her pussy constantly before I subjugated level 1.
I tried using someone elses save and I got the same problem. The thugs will not fuck her pussy anymore, just slap her ass, grab her tits, kiss, but never actually fuck her.
edit: I figured it out. Her endurance was so high, that she wasn't gaining any pleasure. I had to reduce her endurance by removing endurance gear.
Now since you said she wasn't gaining any pleasure, that means she won't get aroused (33% pleasure) and thus she won't be wet, therefore no vaginal sex will happen even if you have pussy and cock desire 100.