How do I get the curse marks?
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The succubi you can encounter in the Sex Club dungeon (and possibly the succubi in the school dungeon) can cast an Estrus curse on you. You cannot get this curse from the curse box.
Getting caught in a trap, in the Sex Club dungeon, can apply a cursed item if you just wait, escaping the trap before it releases you will not apply the cursed item.
Losing to the boss in the Sex Club dungeon will apply a curse mark to you. Repeated losses do not change the curse.
Beating the boss in the sex club dungeon will give you a consumable item, called Cursed Box (in the key items tab), that you can use to apply a curse to yourself.
After you beat the Laboratory dungeon, a vendor will appear in the deep Netherwards that sells the cursed box.